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human renal proximal tubular epithelial cells hk2  (ATCC)


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    Structured Review

    ATCC human renal proximal tubular epithelial cells hk2
    Knockdown of RBCK1 significantly inhibits ccRCC cell proliferation and migration. A Differential expression analysis of RBCK1 between normal and tumor groups. B–D DSS, PFI, and OS survival curves for low- and high-RBCK1 expression subgroups. E Protein expression levels of RBCK1 in normal and tumor groups based on the CPTAC database. F Protein expression levels of RBCK1 in <t>HK2</t> cells and 786-O cells with quantitative analysis (n = 3). G Protein expression levels of RBCK1 in HK2 cells and 769-P cells with quantitative analysis (n = 3). H, I Colony formation assay and Transwell migration assay in 786-O cells (n = 3). J, K Colony formation assay and Transwell migration assay in 769-P cells (n = 3). L, M CCK-8 assay showing cell viability at different time points in 786-O and 769-P cells (n = 3). Data are presented as mean ± SD; significance: * P < 0.05, ** P < 0.01, *** P < 0.001
    Human Renal Proximal Tubular Epithelial Cells Hk2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hk2+human+proximal+tubular+epithelial+cells/HK-2/pmc12976243-93-0-20
    Average 99 stars, based on 4547 article reviews
    human renal proximal tubular epithelial cells hk2 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Comprehensive analysis of PANoptosis-related molecular subtypes and prognostic model development of clear cell renal cell carcinoma"

    Article Title: Comprehensive analysis of PANoptosis-related molecular subtypes and prognostic model development of clear cell renal cell carcinoma

    Journal: Discover Oncology

    doi: 10.1007/s12672-026-04561-9

    Knockdown of RBCK1 significantly inhibits ccRCC cell proliferation and migration. A Differential expression analysis of RBCK1 between normal and tumor groups. B–D DSS, PFI, and OS survival curves for low- and high-RBCK1 expression subgroups. E Protein expression levels of RBCK1 in normal and tumor groups based on the CPTAC database. F Protein expression levels of RBCK1 in HK2 cells and 786-O cells with quantitative analysis (n = 3). G Protein expression levels of RBCK1 in HK2 cells and 769-P cells with quantitative analysis (n = 3). H, I Colony formation assay and Transwell migration assay in 786-O cells (n = 3). J, K Colony formation assay and Transwell migration assay in 769-P cells (n = 3). L, M CCK-8 assay showing cell viability at different time points in 786-O and 769-P cells (n = 3). Data are presented as mean ± SD; significance: * P < 0.05, ** P < 0.01, *** P < 0.001
    Figure Legend Snippet: Knockdown of RBCK1 significantly inhibits ccRCC cell proliferation and migration. A Differential expression analysis of RBCK1 between normal and tumor groups. B–D DSS, PFI, and OS survival curves for low- and high-RBCK1 expression subgroups. E Protein expression levels of RBCK1 in normal and tumor groups based on the CPTAC database. F Protein expression levels of RBCK1 in HK2 cells and 786-O cells with quantitative analysis (n = 3). G Protein expression levels of RBCK1 in HK2 cells and 769-P cells with quantitative analysis (n = 3). H, I Colony formation assay and Transwell migration assay in 786-O cells (n = 3). J, K Colony formation assay and Transwell migration assay in 769-P cells (n = 3). L, M CCK-8 assay showing cell viability at different time points in 786-O and 769-P cells (n = 3). Data are presented as mean ± SD; significance: * P < 0.05, ** P < 0.01, *** P < 0.001

    Techniques Used: Knockdown, Migration, Quantitative Proteomics, Expressing, Colony Assay, Transwell Migration Assay, CCK-8 Assay

    Related Articles

    other:

    Article Title: High glucose couples DJ-1 with PTEN to activate PDGFRβ for renal proximal tubular cell injury
    Article Snippet: We purchased HK2 human proximal tubular epithelial cells from ATCC.

    Article Title: METTL3 aggravates renal fibrogenesis in obstructive nephropathy via the miR-199a-3p/PAR4 axis.
    Article Snippet: Renal fibrosis is among the major factors contributing to the development of chronic kidney disease.. In this regard, although N6-methyladenosine (m6A) modification and micro-RNAs (miRNAs) have been established to play key roles in diverse physiological processes and disease/disorder development, further research is required to identify the probable mechanisms and processes associated with their involvement in renal fibrosis.. In this study, we show that transforming growth factor β1 (TGF-β1)-induced human proximal tubule epithelial cells (HK2) are characterized by dose-dependently higher methyltransferase-like 3 (METTL3) expression.

    Flow Cytometry:

    Article Title: The MIF Receptor CD74 in Diabetic Podocyte Injury
    Article Snippet: .. 3 Culture of HK2 human proximal tubular epithelial cells (ATCC, Rockville, MD) and the assessment of apoptosis as hypodiploid cells by flow cytometry have been described. .. 3 , 29 PD98059 and SB203580 (Stressgen Bioreagent, Ann Arbor, MI) were dissolved in DMSO.

    Article Title: The MIF Receptor CD74 in Diabetic Podocyte Injury
    Article Snippet: .. TNF- (30 ng/ml) was from Immugenex (Los Angeles, CA), and TRAIL (100 ng/ml) was from Alexis (Läufelfingen, Switzerland).3 Culture of HK2 human proximal tubular epithelial cells (ATCC, Rockville, MD) and the as- sessment of apoptosis as hypodiploid cells by flow cytometry have been described.3,29 PD98059 and SB203580 (Stressgen Bioreagent, Ann Arbor, MI) were dissolved in DMSO. ..



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    Knockdown of RBCK1 significantly inhibits ccRCC cell proliferation and migration. A Differential expression analysis of RBCK1 between normal and tumor groups. B–D DSS, PFI, and OS survival curves for low- and high-RBCK1 expression subgroups. E Protein expression levels of RBCK1 in normal and tumor groups based on the CPTAC database. F Protein expression levels of RBCK1 in <t>HK2</t> cells and 786-O cells with quantitative analysis (n = 3). G Protein expression levels of RBCK1 in HK2 cells and 769-P cells with quantitative analysis (n = 3). H, I Colony formation assay and Transwell migration assay in 786-O cells (n = 3). J, K Colony formation assay and Transwell migration assay in 769-P cells (n = 3). L, M CCK-8 assay showing cell viability at different time points in 786-O and 769-P cells (n = 3). Data are presented as mean ± SD; significance: * P < 0.05, ** P < 0.01, *** P < 0.001
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    Image Search Results


    Knockdown of RBCK1 significantly inhibits ccRCC cell proliferation and migration. A Differential expression analysis of RBCK1 between normal and tumor groups. B–D DSS, PFI, and OS survival curves for low- and high-RBCK1 expression subgroups. E Protein expression levels of RBCK1 in normal and tumor groups based on the CPTAC database. F Protein expression levels of RBCK1 in HK2 cells and 786-O cells with quantitative analysis (n = 3). G Protein expression levels of RBCK1 in HK2 cells and 769-P cells with quantitative analysis (n = 3). H, I Colony formation assay and Transwell migration assay in 786-O cells (n = 3). J, K Colony formation assay and Transwell migration assay in 769-P cells (n = 3). L, M CCK-8 assay showing cell viability at different time points in 786-O and 769-P cells (n = 3). Data are presented as mean ± SD; significance: * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Discover Oncology

    Article Title: Comprehensive analysis of PANoptosis-related molecular subtypes and prognostic model development of clear cell renal cell carcinoma

    doi: 10.1007/s12672-026-04561-9

    Figure Lengend Snippet: Knockdown of RBCK1 significantly inhibits ccRCC cell proliferation and migration. A Differential expression analysis of RBCK1 between normal and tumor groups. B–D DSS, PFI, and OS survival curves for low- and high-RBCK1 expression subgroups. E Protein expression levels of RBCK1 in normal and tumor groups based on the CPTAC database. F Protein expression levels of RBCK1 in HK2 cells and 786-O cells with quantitative analysis (n = 3). G Protein expression levels of RBCK1 in HK2 cells and 769-P cells with quantitative analysis (n = 3). H, I Colony formation assay and Transwell migration assay in 786-O cells (n = 3). J, K Colony formation assay and Transwell migration assay in 769-P cells (n = 3). L, M CCK-8 assay showing cell viability at different time points in 786-O and 769-P cells (n = 3). Data are presented as mean ± SD; significance: * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: Human renal proximal tubular epithelial cells HK2, human ccRCC 786-O cells, and human ccRCC 769-P cells were obtained from the American Type Culture Collection (ATCC, USA).

    Techniques: Knockdown, Migration, Quantitative Proteomics, Expressing, Colony Assay, Transwell Migration Assay, CCK-8 Assay